Dr. Bacher is post-doctoral fellow in the Department of Psychiatry at the University of Toronto and Neurochemical Imaging, Addiction Psychiatry and Schizophrenia Programs at the Centre for Addiction and Mental Health (CAMH) in Toronto, Ontario, Canada. Ms. Rabin and Ms. Woznica are research analysts at the CAMH. Dr. Sacco is research affiliate in the Department of Psychiatry at Yale University School of Medicine in New Haven, Connecticut. Dr. George is professor and chair in Addiction Psychiatry and Clinical Director of the Schizophrenia Program in the Department of Psychiatry at the University of Toronto and Addiction Psychiatry and Schizophrenia Programs at the CAMH.
Disclosures: Dr. Bacher is fellowship recipient of the 2009 National Alliance for Research on Schizophrenia and Depression (NARSAD) Young Investigator Award from the Tobacco Use in Special Populations program at the Centre for Addiction and Mental Health. Ms. Rabin and Ms. Woznica report no affiliation with or financial interest in any organization that may pose a conflict of interest. Dr. Sacco receives grant support from the 2005 NARSAD Young Investigator Award. Dr. George is consultant to the Canada Foundation for Innovation (CFI), the Canadian Institutes for Health Research (CIHR), the Donaghue Foundation for Medical Research, Eli Lilly, Evotec, GlaxoSmithKline, Janssen-Ortho, Memory Pharmaceuticals, Pfizer, Prempharm, sanofi-aventis, Sepracor, and Targacept, Inc.; is on the advisory boards of Pfizer and Sepracor; receives honoraria from Eli Lilly, Evotec, GlaxoSmithKline, Janssen-Ortho, Memory Pharmaceuticals, Pfizer, and Prempharm; and receives grant support from the CFI, the CIHR, the Donaghue Foundation for Medical Research, the NARSAD, the National Institute on Drug Abuse, sanofi-aventis, Sepracor, and Targacept, Inc.
Please direct all correspondence to: Tony P. George, MD, FRCPC, Department of Psychiatry, University of Toronto and Addiction Psychiatry and Schizophrenia Programs, Centre for Addiction and Mental Health (CAMH), 250 College Street, Room CS 734, Toronto, ON, Canada, M5T1R8; Tel: 416-535-8501 x4544; Fax: 416-979-4676; E-mail: firstname.lastname@example.org.
• There is increasing evidence that nicotinic acetylcholine receptors are dysregulated in several neuropsychiatric disorders.
• This pathology may be related to the high prevalence of tobacco dependence in several of these disorders.
• These findings have tremendous implications for the development of nicotinic agents for the treatment of clinical and cognitive symptoms associated with these disorders.
Nicotinic acetylcholine receptor (nAChR) dysfunction is believed to contribute to numerous neurpsychiatric disorders. nAChRs belong to the class of ligand-gated ion channels that are present in the central nervous system. The endogenous ligand for nAChRs is acetylcholine, and nicotine directly acts on this receptor. nAChR modulation may play a modulatory role in several neuropsychiatric disorders. It may improve clinical features such as depressive symptoms; parkinsonism; and cognitive dysfunction related to working and verbal memory, executive functions, and attention. This article discusses nAChR modulation and drugs that act on the nAChR as an agonist, antagonist, or partial agonist in neuropsychiatric disorders and potential therapeutic implications in a variety of “nicotine-responsive” neuropsychiatric disorders.
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels in the central nervous system and consist of α and β subunits. Twelve subtypes of the nAChR have been identified; the α4β2 subunit combination is the most common.1 nAChRs are found in thalamus, basal ganglia, cerebral cortex, hippocampus, and cerebellum.2 Acetylcholine (Ach) is the endogenous ligand of the nAChR. Nicotine is the main addictive compound in tobacco. nAChR activation by nicotine is time and dose dependent. Chronic nicotine administration causes desensitization, inactivation, and upregulation of nAChRs, in contrast to the effects of typical agonist drugs.3 There are two classes of central nAChRs. First, the high-affinity nAChR, a heteromer of α and β subunits, is blocked by the antagonists mecamylamine and dihydro-β-erythroidine, and at low doses is stimulated by the nAChR partial agonist varenicline. Second, the low-affinity nAChR is a α subunit homopentamer and can be inhibited by the snake poison α-bungarotoxin and the antagonist methyllycaconitine. Activation of brain nAChRs by ACh, nicotine, or varenicline (the smoking cessation medication) binding causes an increase of metabolism and release of neurotransmitters like dopamine (DA), serotonin (5-HT), norepinephrine (NE), γ-aminobutyric acid, and opioid peptides.4-6 DA plays a crucial role in the mesolimbic reward systems of drugs of abuse and the dopaminergic and serotonergic systems are involved in mood disorders.7
In North America, tobacco smoking prevalence in neuropsychiatric disorders is up to five times higher and smoking cessation rates 33% to 50% of those found in the general population.1,7-9 Nicotine and cigarette smoking modulation of clinical and cognitive symptoms differs between patients with neuropsychiatric disorders and in healthy controls. This may be due, in part, to genetic or biochemical differences in nAChR systems between healthy individuals and people with neuropsychiatric disorders.
This article reviews evidence highlighting the involvement of nAChR systems in neuropsychiatric disorders, and discusses the potential application of agents which modulate nAChR function for the treatment of these disorders.
Evidence for Nicotinic Receptor Modulation Specific to Neuropsychiatric Disorders: Therapeutic Implications
Schizophrenia is a neuropsychiatric disorder characterized by deficits in neurocognition, hallucinations (primary auditory), and delusions.10 Post-mortem studies suggest a dysregulation of both high- and low-affinity nAChR systems, with low levels of the nAChR subtypes (α and β) in hippocampus and frontal cortex.11-13 A link between schizophrenia and abnormalities of the α7 gene was observed in numerous studies14-17; no association was found between variations in the α2 nAChR gene and schizophrenia.18 Abnormalities in central dopamine systems are proposed in schizophrenia, including hyperfunctional subcortical, and hypofunctional prefrontal cortex DA system activities.19 It is hypothesized that the DA deficit in the cortex causes mesolimibic DA hyperactivity, leading in positive, negative, and cognitive symptoms of schizophrenia.19,20 Administration of nicotine and tobacco smoking ameliorates cognitive deficits in individuals with schizophrenia.21,22 A double-blind, randomized cross-over design (n=12) was used to deternimine the effectiveness of 3[(2,4-dimethoxy)-benzylidene]-anabaseine (DMXB-A), a partial a7 nicotinic cholinergic agonist and a weak antagonist at α4β2 nAChRs and serotonin 5-HT3 receptors on cognition in non smokers with schizophrenia.23 Administration of a single dose of DMXB-A, a natural alkaloid derivative, significantly improved cognition using the Repeatable Battery for the Assessment of Neuropsychological Status total scale score and P50 inhibition.24
In another study,25 a dose of 150 mg BID DMXB-A was administered to 31 subjects with a diagnosis of schizophrenia and led to significant improvements on the Assessment of Negative Symptoms (SANS) total score. No improvements were observed in the Brief Psychiatric Rating Scale and SANS when given 75 mg. This was measured in a 4-week long placebo-controlled, double-blind, cross-over phase 2 study.25 A partial and potential agonist on human α7 receptor JN403 ((S)-(1-Aza-bicyclo[2.2.2]oct-3-yl)-carbamic acid (S)-1-(2-fluoro-phenyl)-ethyl ester) ameliorated cognition, sensory gating, epilepsy, and pain in animal models.26 This agent has yet to be tested in the human population.
Antipsychotics (eg, haloperidol) can induce side effects like cognitive and sensory gating deficits, and it is hypothesized that people with schizophrenia may smoke to remedy their cognitive deficits and antipsychotic-induced side effects.27,28 Switching patients from first-generation (eg, haloperidol) to second-generation antipsychotics (eg, clozapine) can reduce cigarette smoking29-31 and may facilitate smoking cessation with standard treatments like nicotine-replacement therapy (NRT) and bupropion.22,32 George and colleagues33 demonstrated that several weeks of smoking abstinence can cause disruption of cognitive functioning, such as spatial work memory deficits, in smokers with schizophrenia. Nicotine administration through smoking increases working memory in smokers with schizophrenia; this phenomenon is not observed in non-psychiatric smokers.34 One small open-label study35 suggested that the α4β2 partial agonist and α7 full agonist varenicline, a Food and Drug Administration-approved agent for smoking-cessation treatment in adults, may be particularly effective for smoking cessation in schizophrenia.
Among mood disorders, major depressive disorder (MDD) and bipolar disorder are the most common diagnoses. In contrast to MDD, where people experience low and depressed mood, patients with bipolar disorder experience both depression and mania.36 Hypercholinergic neurotransmission is associated with depressed mood and mediated through excessive nAChR activation.37,38 Antidepressants fluoxetine and bupropion have nAChR antagonist properties and may act in part by normalizing hypercholinergic tone present in depressed states in addition to their monoamine reuptake inhibitory properties.39 Mecamylamine has an antidepressant effect in wild type mice but has a lack of effect in α7 or β2 knock-out mice and potentiates the antidepressant activity of amitriptyline in rodents.40,41 An antidepressant effect of mecamylamine was also confirmed in two preliminary studies in depressed patients, but further controlled studies are still warranted.42,43 Central 5-HT levels are low in MDD, and nicotine causes a release of 5-HT, which might, in part, explain the high smoking prevalence in people with depression.8 Results from studies in depressed non-smokers using transdermal nicotine were not supportive of the hypothesis that nicotine itself exerts antidepressant effects.45,46 In contrast, self-administration of nicotine appears to improve depression-prone smokers’ emotional response to a pleasant stimulus.46 Typical symptoms during smoking withdrawal include depression, anxiety, and nervousness, among others, whereas depressive mood predicts higher withdrawal symptoms.47 NRT for smoking cessation in history-positive depressed smokers, however, was almost as successful as in healthy smokers.48 Varenicline has shown antidepressant effects in animal models.49 Varenciline was recently tested in an 8-week open-label trial. Fourteen (87%) out of 18 depressed subjects completed the study and led to a significant improvement in depression symptoms. Reasons for dropout were gastro-intestinal side effects (n=3) and worsening of mood (n=1).50
Little evidence for the involvement of the nAChR system in bipolar disorder is available. Administration of mecamylamine stabilized the mood of two individuals suffering from Tourette’s syndrome with comorbid bipolar depression.51 A genetic study found no association between bipolar disorder and the α2 nAChR subunit gene.52
Attention-deficit/hyperactivity disorder (ADHD) is a highly prevalent, worldwide disorder estimated to affect 5% to 10% of children53 and 3% to 6% of adults.54 ADHD is characterized by a persistent pattern of inattention as well as distractibility and/or hyperactivity to the extent that it comprises academic or occupational functioning.55 Longitudinal ADHD studies in youths demonstrate that while hyperactivity and impulsivity symptoms decrease over time, inattention tends to persist.56 An estimated 50% of adults with ADHD have clinically relevant levels of hyperactivity and impulsivity while >90% have prominent attentional symptoms.57 Moreover, individuals with prominent cognitive impairments are at a greater risk for more academic and occupational difficulties.58
Theories of the neurobiologic basis of ADHD have largely focused on the dysregulation of catecholamine systems. The primary pharmacotherapy approach for ADHD is the prescription of psychostimulants such as methylphenidate and amphetamines, which enhance activity of DA and NE, resulting in reduced symptomatology. However, it has been proposed that other neurotransmitter systems may be implicated in the specific cognitive deficits of ADHD.
Studies investigating nicotinic agents in individuals with ADHD have shown promising symptomatic and cognitive improvements. Levin and colleagues59 examined the acute effects of transdermal nicotine in adults smokers and non-smokers with ADHD and found significant improvements in self-rated vigor, concentration, and observer-rated severity illness in both groups. McClernon and colleagues60 reported that cognitive processes, especially those associated with reaction time variability, are more disrupted in smokers with ADHD following smoking abstinence, compared to non-ADHD smokers. Nicotine treatment has also been shown to normalize inhibitory behavior in people with ADHD.61
A preliminary study62 of 10 smokers with ADHD receiving nicotine patch, methylphenidate, or combination of the two, showed that nicotine patches and stimulant medication alone and in combination reduced difficulty concentrating and core ADHD symptoms compared with placebo patch only. Borderline improvement in impatience and self-control was seen with nicotine patch administration. Wilens and colleagues63 studied the nicotinic agonist ABT-418, selective for the high-affinity nAChR, in 32 adults with ADHD. Significant improvements in subjective ratings of attentiveness and observer-rated severity illness were observed following treatment. Wilens and colleagues64 tested ABT-089, a newer more selective high-affinity nAChR agonist, in adults with ADHD. The drug was administered in a multi-dose, randomized, double-blind, placebo-controlled trial and results indicated greater improvements in symptoms scores, ADHD index hyperactive/impulsive ratings, and clinical global impression. These findings suggest that stimulation of nicotinic cholinergic receptors do not only target cognitive domains but also address the overt behavioral symptoms of ADHD. Further studies are necessary to clarify if nicotine’s effects on cognition in ADHD are mediated by cholinergic systems or cholinergic modulation of dopamine function.
Autistic individuals are, in stark contrast to individuals with ADHD, hyper-focused. Core symptoms include deficits in all aspects of social reciprocity—pragmatic communication deficits, language delays, and behavioral problems.65 Autopsy studies66,67 show depletion of nAChRs in certain areas of the cortex, cerebellum, and thalamus, which are involved in attention and sensory processes in autism. A decrease in three of the four nicotinic receptor subtypes in the cerebellum was observed.68 Some authors69 hypothesize that selective antagonism of neuronal nAChRs may ameliorate the core symptoms of autism by normalization of cholinergic tone. Children and adolescents with autism were given the acetylcholinesterase inhibitor (AChEI) donezepil in one open-label trial over 2 months. Decreases in the irritability and hyperactivity were observed, but no changes in inappropriate speech, lethargy, and stereotypies. Lack of a significant effect could be explained by the low number of study participants (n=8) and the concomitant psychoactive medications they received.70 Results from a case study71 in three adults diagnosed with autism showed that galantamine (a competitive, reversible AChEI and an allosteric potentiating ligand for nAChRs) might increase expressive language and communication. A significant reduction in parent-rated irritability and social withdrawal as well as significant improvements in emotional lability and inattention was measured in an open-label study72 where 13 children and adolescents were given galantamine over 12 weeks. The dual-action AChEI rivastigmine tartrate enhanced significantly expressive speech and overall autistic behavior in 32 autistic children in on open-label 12-week study.73 Further studies are needed to elucidate the role of the cholinergic system in autism.
Alzheimer’s disease is the most common neurodegenerative disorder and the most common type of dementia in the elderly.74 Patients with Alzheimer’s disease show a deficit in cholinergic innervation in hippocampus and cerebral cortex with a significant reduction in choline acetyltransferase activity in these regions.75-77 Pharmacologic compounds used for treatment of Alzheimer’s disease are AChEIs such as physostigmine or donazepil. A loss of α4β2 nAChR as well as a neurotoxic effect of β-amyloid peptides in neurotic plaques on α7 nAChRs were observed.78 Newhouse and colleagues79 demonstrated that intravenously administered nicotine (.125, .25 and .50 μg/kg/min) produced dose-dependent improvement in intrusion errors on a word recall task in non-smokers with Alzheimer’s disease, and that maximum effects occurred at .25 μg/kg/min, suggesting an “inverted-U” dose-response pattern. Administration of transdermal nicotine improved performance on a repeated acquisition task in patients with probable Alzheimer’s disease,80 however, other studies have not supported this finding since performance on a letter memory test did not improve with transdermal nicotine patch (TNP). A study by White and colleagues81 all found that performance on a letter memory test did not improve with TNP. Using a within-subjects placebo-controlled study design of three doses of the nAChR channel activator ABT-418 in patients with moderate Alzheimer’s disease, Potter and colleagues82 demonstrated that this agent improved dose-dependently deficits in total recall in a verbal learning task, a seven item selective reminding task, and in non-verbal tasks such as spatial learning and memory and repeated acquisition. Methodologic differences amongst these studies, including dose and route of nicotine administration, may explain these disparate effects of nicotine on learning and memory in Alzheimer’s disease.
In the domain of attention, one study83 found that intravenous nicotine improved detection performance on the critical flicker fusion test in patients with Alzheimer’s disease, and improved their ability to discriminate stimuli and their reaction times, suggesting effects of nicotine on visual perceptual and attentional cortical mechanisms. Similarly, perception in patients with Alzheimer’s disease improved on the flicker fusion task in response to subcutaneous nicotine administration.84 Thus, nicotine administration appears to improve some forms of attentional function in Alzheimer’s disease.
Several epidemiologic studies85-87 of Alzheimer’s disease suggested a possible protective effect of cigarette smoking. However, other authors88 suggested that cigarette smokers are more likely to develop Alzheimer’s disease compared to those who have no smoking history. Data regarding the correlation between Alzheimer’s disease and smoking are conflicting, probably due to methodologic errors in case-control studies.
Memantine is an FDA-approved agent for the treatment of Alzheimer’s disease symptoms.89 Its main mechanism of action is the non-competitive blockage of central NMDA receptors. However, studies90,91 in cell cultures like HEK or K177 show that memantine also has a non-competitive antagonistic effect on the α7 nicotine receptor.
(E)-3-(2,4-dimethoxybenzylidene)-3,4,5,6-tetrahydro-2,3’-bipyridine dihydrochloride, an experimental α7 agonist, improved attention and memory in 16 healthy subjects in a randomized, placebo-controlled, double-blind, multiple dose study92 and could be considered as a novel treatment for dementia. Another experimental compound, the positive allosteric modulator of the α7 nAChR, 1-(5-chloro-2-hydroxy-phenyl)-3-(2-chloro-5-trifluoromethyl-phenyl)-urea, was tested so far in rodents and shows cognition-enhancing properties.93
Parkinson’s disease is a neurodegenerative disorder characterized by slowed processing speed, abnormal visuospatial processing, and central motor dysfunction such as muscular rigidity, tremor, and sustaining movement.94 nAChR activation plays a crucial role in regulating striatal dopaminergic function, and these dopaminergic systems are critical in motor control, as evidenced by findings that their disruption results in movement disorders such as Parkinson’s disease.95 Symptoms of Parkinson’s disease are a result of the loss of nicotinic binding sites and degeneration of dopaminergic neurons in part of the midbrain known as substantia nigra, and the neuronal degeneration is paralleled by the loss of high-affinity nicotine-binding sites.96 Even moderate lesions in the striatum lead to a decrease in α6β2 nAChRs. In contrast, α4β2 subtypes are affected only in severe degeneration and α7 nAChRs seem to be to some extent affected.95 Human binding and immunoprecipitation studies demonstrate that the decrease in α6β2 nAChRs are significantly greater than in α4β2 receptors in some striatal regions.97-99 The surviving DA neurons contain inclusions called Lewy bodies and Lewy neurites.100 Fibrillation of α-synuclein, a compound found in Lewy bodies and Lewy neurites, plays a crucial role in the pathogenesis of Parkinson’s disease. Nicotine blocks this fibrillation and stimulates striatal dopamine neurons that are damaged in Parkinson’s disease.101,102 Nicotine treatment enhances expression of some nAChR subtypes decreased with nigrostriatal damage, which may suggest that function mediated through these receptors is restored closer to control levels with nicotine treatment.103 Indeed, nicotine administration improves extrapyramidal symptoms and some cognitive function in Parkinson’s disease patients.104-106 However, there are also studies107-109 demonstrating no improvement or even worsening of Parkinson’s disease symptoms under nicotine.
Results from >50 studies show that smokers are protected against Parkinson’s disease.102 One explanation could be the attribution metabolites of nicotine, like cotinine and nornicotine. Cotinine exhibited a non-receptor mediated cytoprotective effect and nornicotine reduced β-amyloid aggregation in in vitro studies.110,111 Nicotine also ameliorates L-dopa- (the gold standard for the treatment of Parkinson’s disease) induced dyskinesia in non-human primates.112 Still, the underlying mechanism between the neuroprotective effects of smoking and nicotine in Parkinson’s disease needs further investigation. In 77 patients with early Parkinson’s disease, no antiparkinsonian or cognitive-enhancing effects were demonstrated in a placebo-controlled, double-blind study113 with the non-approved selective α4β2 nicotinic agonist SIB-5008Y (altinicline; (S)-(-)-5-ethynyl-3-(1-methyl-2-pyrrolildinyl) pyridine maleate). Galantamine synergistically enhances the neuroprotective effect of nicotine against dopaminergic neuronal loss through an allosteric modulation of α7 nAChR activation in rodents.114 Accordingly, agonists on nAChR may represent a new approach in the treatment of Parkinson’s disease due to their potential neuroprotective effect.
Tourette’s syndrome is a hyperkinetic disorder, characterized by the presence of involuntary motor and verbal tics that first manifest in childhood. Tourette’s syndrome is commonly associated with other learning and behavioral difficulties. Its pathogenesis is unknown.115 Tourette’s syndrome is frequently treated with haloperidol, a typical antipsychotic and dopamine antagonist. Studies suggest that neuronal nAChRs are effective in mediating the symptoms of Tourette’s syndrome.116 Demonstrations with laboratory rats showed that low doses of nicotine administered chronically can potentiate the cataleptic effect induced by haloperidol.117 It was hypothesized that this effect could be replicated in humans, independent of their smoking status. The combination of transdermal nicotine and haloperidol significantly reduced tic frequency and severity, compared to neuroleptic treatment alone.118,119 Nicotine gum was also shown to augment haloperidol treatment, compared to placebo gum which had no effects. However, nicotine gum was only effective during the period of chewing.120 Long-term benefits of the transdermal nicotine patch have been reported. Shytle and colleagues121 found that each application of a single transdermal nicotine patch (7 mg/24 hour) produced a significant reduction in tics for ~1–2 weeks following application, as measured by the Yale Global Tic Severity Scale. Nicotine also appears to improve Tourette’s syndrome symptomatology in the absence of neuroleptics. The use of nicotinic antagonist treatment for Tourette’s syndrome has also been proposed. However, the classic nAChR antagonist mecamylamine administered as a monotherapy had no effect on tics in an 8-week trial.122
The comorbidity of psychiatric disorders and tobacco dependence is high when compared with the average population, and the biologic significance of this comorbid association suggests that therapeutic approaches that treat the underlying neuropsychiatric illness should be taken. This proof of concept has been well described in the literature under the auspices of “nicotine-responsive neuropsychiatric illness” (Table 1).123,124 Moreover, smoking cessation is more difficult in many of these patient groups because of the intrinsic dysregulation in nAChR systems associated with this disorder (eg, schizophrenia, mood disorders, Tourette’s syndrome, ADHD), but in some cases this nAChR dysregulation may actually protect against the initiation and maintenance of nicotine addiction (eg, Alzheimer’s disease, Parkinson’s disease, autism). Accordingly, further research is needed to parse underlying mechanisms that confer vulnerability to tobacco addiction in these populations, and to determine the optimal strategy to use nAChR-based therapeutics to treat these “nicotine-responsive” neuropsychiatric disorders. Since prolonged treatment with nicotine leads to nAChR desensitization, there is now clear evidence that administration of nAChR antagonists (eg, mecamylamine) could have therapeutic value in the treatment of several disorders, including Tourette’s syndrome123 and MDD.42 Several available nicotinic agonists (including allosteric moduators such as galantamine, and partial agonists such as varenicline) and others in development (eg, the α7 nAChR partial agonist DMXB-A, and the α4β2 nAChR agonists TC-1707 and ABT-089) could have enormous potential in treatment of such “nicotine-responsive” illnesses (Table 2). Additional studies in animal models are required to provide more insight into the underlying mechanisms of nAChR system. Given the tremendous promise of agents treating these neuropsychiatric illnesses, it has been suggested to develop academic-industry partnerships in order to identify and screen nicotinic agents that are safe for human use and carry the most therapeutic potential. Further pre-clinical and translational research is warranted in order to understand the biobehavioral mechanisms that will guide such medications development. PP
1. McGehee DS, Role LW. Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons. Annu Rev Physiol. 1995;57:521-546.
2. Brody A, Mandelkern, MA, Jarvik, ME, et al. Differences between smokers and nonsmokers in regional gray matter volumes and densities. Biol Psychiatry. 2004;55(1):77-84.
3. Picciotto M, Addy, NA, Mineur, YS, Brunzell, DH. It is not “either/or”: activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood. Prog Neurobiol. 2008;84(4):329-342.
4. Mansvelder H, McGehee, DS. Cellular and synaptic mechanisms of nicotine addiction. J Neurobiol. 2002;53(4):606-617.
5. McGehee D, Heath MJ, Gelber S, Devay P, Role LW. Nicotine enhancement of fast excitatory synaptic transmission in CNS by presynaptic receptors. Science. 1995;269(5231):1692-1696.
6. Pich E, Pagliusi SR, Tessari M, Talabot-Ayer D, Hooft van Huijsduijnen R, Chiamulera C. Common neural substrates for the addictive properties of nicotine and cocaine. Science. 1997;275(5296):83-86.
7. Morisano D, Bacher I, Audrain-McGovern J, George TP. Mechanisms Underlying the Co-morbidity of Tobacco Use in Mental Health and Addictive Disorders. Can J Psychiatry. 2009;54(6):356-367.
8. Kalman D, Morissette SB, George TP. Co-morbidity of smoking in patients with psychiatric and substance use disorders. Am J Addict. 2005;14(2):106-123.
9. Hitsman B, Moss TG, Montoya ID, George T.. Treatment of tobacco dependence in mental health and addictive disorders. Can J Psychiatry. 2009;54(6):368-378.
10. Freedman R. Schizophrenia. N Engl J Med. 2003;349(18):1738-1749.
11. Breese C, Lee MJ, Adams CE, et al. Abnormal regulation of high affinity nicotinic receptors in subjects with schizophrenia. Neuropsychopharmacology. 2000;23(4):351-364.
12. Freedman R, Hall M, Adler LE, Leonard S. Evidence in postmortem brain tissue for decreased numbers of hippocampal nicotinic receptors in schizophrenia. Biol Psychiatry. 1995;38(1):22-33.
13. Martin-Ruiz C, Haroutunian VH, Long P, et al. Dementia rating and nicotinic receptor expression in the prefrontal cortex in schizophrenia. Biol Psychiatry. 2003;54(11):1222-1233.
14. De Luca V, Likhodi O, Van Tol HH, Kennedy JL, Wong AH. Regulation of alpha7-nicotinic receptor subunit and alpha7-like gene expression in the prefrontal cortex of patients with bipolar disorder and schizophrenia. Acta Psychiatr Scand. 2006;114(3):211-215.
15. Gault J, Hopkins J, Berger R, et al. Comparison of polymorphisms in the alpha7 nicotinic receptor gene and its partial duplication in schizophrenic and control subjects. Am J Med Genet B Neuropsychiatr Genet. 2003;123(1):39-49.
16. Leonard S, Gault J, Hopkins J, et al. Association of promoter variants in the alpha7 nicotinic acetylcholine receptor subunit gene with an inhibitory deficit found in schizophrenia. Arch Gen Psychiatry. 2002;59(12):1085-1096.
17. Mathew S, Law AJ, Lipska BK, et al. Alpha7 nicotinic acetylcholine receptor mRNA expression and binding in postmortem human brain are associated with genetic variation in neuregulin 1. Hum Mol Genet. 2007;16(23):2921-2932.
18. Blaveri E, Kalsi G, Lawrence J, et al. Genetic association studies of schizophrenia using the 8p21–22 genes: prepronociceptin (PNOC), neuronal nicotinic cholinergic receptor alpha polypeptide 2 (CHRNA2) and arylamine N-acetyltransferase 1 (NAT1). Eur J Hum Genet. 2001;6:469-472.
19. Knable MB, Weinberger DR. Dopamine, the prefrontal cortex and schizophrenia. J Psychopharmacol. 1997;11(2):123-131.
20. Dalack GW, Healy DJ, Meador-Woodruff JH. Nicotine dependence in schizophrenia: clinical phenomena and laboratory findings. Am J Psychiatry. 1998;155(11):1490-1501.
21. Dépatie L, O’Driscoll GA, Holahan AL, Atkinson V, Thavundayil JX, Kin NN, Lal S. Nicotine and behavioral markers of risk for schizophrenia: a double-blind, placebo-controlled, cross-over study. Neuropsychopharmacol. 2002;27(6):1056-1070.
22. George T, Vessicchio JC, Termine A, et al. Effects of smoking abstinence on visuospatial working memory function in schizophrenia. Neuropsychopharmacol. 2002;26(1):75-85.
23. Kem W, Mahnir VM, Prokai L, et al. Hydroxy metabolites of the Alzheimer’s drug candidate 3-[(2,4-dimethoxy)benzylidene]-anabaseine dihydrochloride (GTS-21): their molecular properties, interactions with brain nicotinic receptors, and brain penetration. Mol Pharmacol. 2004;65(1):56-67.
24. Olincy A, Harris JG, Johnson LL, et al. Proof-of-concept trial of an alpha7 nicotinic agonist in schizophrenia. Arch Gen Psychiatry. 2006;63(6):630-638.
25. Freedman R, Olincy A, Buchanan RW, et al. Initial phase 2 trial of a nicotinic agonist in schizophrenia. Am J Psychiatry. 2008;165(8):1040-1047.
26. Feuerbach D, Lingenhoehl K, Olpe HR, et al. The selective nicotinic acetylcholine receptor alpha7 agonist JN403 is active in animal models of cognition, sensory gating, epilepsy and pain. Neuropharmacology. 2009;56(1):254-263.
27. Goff D, Henderson DC, Amico E. Cigarette smoking in schizophrenia: relationship to psychopathology and medication side effects. Am J Psychiatry. 1992;149(9):1189-1194.
28. Kumari V, Postma P. Nicotine use in schizophrenia: the self medication hypotheses. Neurosci Biobehav Rev. 2005;29(6):1021-1034.
29. McEvoy J, Brown S. Smoking in first-episode patients with schizophrenia. Am J Psychiatry. 1999;156(7):1120-1121.
30. Green AI. Treatment of schizophrenia and comorbid substance abuse: pharmacologic approaches. J Clin Psychiatry. 2006;67(suppl 7):31-37.
31. George TP, Sernyak MJ, Ziedonis DM, Woods SW. Effects of clozapine on smoking in chronic schizophrenic outpatients. J Clin Psychiatry. 1995;56(8):344-346.
32. Gonzales D, Rennard SI, Nides M, et al. Varenicline, an alpha4beta2 nicotinic acetylcholine receptor partial agonist, vs sustained-release bupropion and placebo for smoking cessation: a randomized controlled trial. JAMA. 2006;296(1):47-55.
33. George TP, Vessicchio J, Termine A, et al. Effects of smoking abstinence on visuospatial working memory function in schizophrenia. Neuropsychopharmacology. 2002;26(1):75-85.
34. Sacco K, Termine A, Seyal A, et al. Effects of cigarette smoking on spatial working memory and attentional deficits in schizophrenia: involvement of nicotinic receptor mechanisms. Arch Gen Psychiatry. 2005;62(6):649-659.
35. Evins A, Culhane MA, Alpert JE, et al. A controlled trial of bupropion added to nicotine patch and behavioral therapy for smoking cessation in adults with unipolar depressive disorders. J Clin Psychopharmacol. 2008;28(6):660-666.
36. First M, Spitzer R, Williams J, et al. Structured Clinical Interview for DSM-IV-Non-Patient Edition (SCID-NP, Version 1.0). Washington, DC: American Psychiatric Press; 1995.
37. Janowsky D, el-Yousef MK, Davis JM, Sekerke HJ. A cholinergic-adrenergic hypothesis of mania and depression. Lancet. 1972;2(7778):632-635.
38. Janowsky D, el-Yousef MK, Davis JM, Hubbard B, Sekerke HJ. Cholinergic reversal of manic symptoms. Lancet. 1972;1(7762):1236-1237.
39. Shytle RD SA, Lukas RJ, Newman MB, Sheehan DV, Sanberg PR. Nicotinic acetylcholine receptors as targets for antidepressants. Mol Psychiatry. 2002;7(6):525-535.
40. Caldarone B, Harrist A, Cleary MA, Beech RD, King SL, Picciotto MR. High-affinity nicotinic acetylcholine receptors are required for antidepressant effects of amitriptyline on behavior and hippocampal cell proliferation. Biol Psychiatry. 2004;56(9):657-664.
41. Rabenstein R, Caldarone BJ, Picciotto MR. The nicotinic antagonist mecamylamine has antidepressant-like effects in wild-type but not beta2- or alpha7-nicotinic acetylcholine receptor subunit knockout mice. Psychopharmacology. 2006;189(3):395-401.
42. George TP, Sacco KA, Vessicchio JC, Weinberger AH, Shytle RD. Nicotinic antagonist augmentation of selective serotonin reuptake inhibitor-refractory major depressive disorder: a preliminary study. J Clin Psychopharmacol. 2008;28(3):340-344.
43. Shytle R, Silver AA, Sheehan KH, Sheehan DV, Sanber, PR. Neuronal nicotinic receptor inhibition for treating mood disorders: preliminary controlled evidence with mecamylamine. Depress Anxiety. 2002;16(3):89-92.
44. McClernon F, Hiott FB, Westman EC, Rose JE, Levin ED. Transdermal nicotine attenuates depression symptoms in nonsmokers: a double-blind, placebo-controlled trial. Psychopharmacology (Berl). 2006189(1):125-133.
45. Salín-Pascual R, Rosas M, Jimenez-Genchi A, Rivera-Meza BL, Delgado-Parra V. Antidepressant effect of transdermal nicotine patches in nonsmoking patients with major depression. J Clin Psychiatry. 1996;57(9):387-389.
46. Spring B, Cook JW, Appelhans B, et al. Nicotine effects on affective response in depression-prone smokers. Psychopharmacology. 2008;196(3):461-471.
47. Cook J, Spring B, McChargue DE, et al. Influence of fluoxetine on positive and negative affect in a clinic-based smoking cessation trial. Psychopharmacology (Berl). 2004;173(1-2):153-159.
48. Kinnunen T, Korhonen T, Garvey AJ. Role of nicotine gum and pretreatment depressive symptoms in smoking cessation: twelve-month results of a randomized placebo controlled trial. Int J Psychiatry Med. 2008;38(3):373-389.
49. Mineur Y, Somenzi O, Picciotto MR. Cytisine, a partial agonist of high-affinity nicotinic acetylcholine receptors, has antidepressant-like properties in male C57BL/6J mice. Neuropharmacology. 2007;52(5):1256-1262.
50. Philip N, Carpenter LL, Tyrka AR, Whiteley LB, Price LH. Varenicline augmentation in depressed smokers: an 8-week, open-label study. J Clin Psychiatry. 2009;70(7):1026-1031.
51. Shytle R, Silver AA, Sanberg PR. Comorbid bipolar disorder in Tourette’s syndrome responds to the nicotinic receptor antagonist mecamylamine (Inversine). Biol Psychiatry. 2000;48(10):1028-1031.
52. Lohoff F, Ferraro TN, McNabb L, et al. No association between common variations in the neuronal nicotinic acetylcholine receptor alpha2 subunit gene (CHRNA2) and bipolar I disorder. Psychiatry Res. 2005;135(3):171-177.
53. Biederman J. Attention-deficit/hyperactivity disorder: a selective overview. Biol Psychiatry. 2005;57(11):1215-1220.
54. Faraone SV, Biederman J. What is the prevalence of adult ADHD? Results of a population screen of 966 adults. J Atten Disord. 2005;9(2):384-391.
55. Diagnostic and Statistical Manual of Mental Disorders. 4th ed. Washington, DC: American Psychiatric Association; 1994.
56. Achenbach TM, Howell CT, McConaughy SH, Stanger C. Six-year predictors of problems in a national sample: IV. Young adult signs of disturbance. J Am Acad Child Adolesc Psychiatry. 1998;37(7):718-727.
57. Millstein RB, Wilens TE, Biderman J, Spencer TJ. Presenting ADHD symptoms and subtypes in clinically referred adults with ADHD. J Atten Disord. 1997;2(3):159-166.
58. Faraone SV, Biederman J, Monuteaux MC. Attention deficit hyperactivity disorder with bipolar disorder in girls: further evidence for a familial subtype? J Affect Disord. 2001;64(1):19-26.
59. Levin ED, Conners CK, Sparrow E, et al. Nicotine effects on adults with attention-deficit/hyperactivity disorder. Psychopharmacology (Berl). 1996;123(1):55-63.
60. McClernon FJ, Kollins SH, Lutz AM, et al. Effects of smoking abstinence on adult smokers with and without attention deficit hyperactivity disorder: results of a preliminary study. Psychopharmacology (Berl). 2008;197(1):95-105.
61. Potter AS, Newhouse PA. Effects of acute nicotine administration on behavioral inhibition in adolescents with attention-deficit/hyperactivity disorder. Psychopharmacology (Berl). 2004;176(2):182-194.
62. Gehricke JG, Whalen CK, Jamner LD, Wigal TL, Steinhoff K. The reinforcing effects of nicotine and stimulant medication in the everyday lives of adult smokers with ADHD: a preliminary examination. Nicotine Tob Res. 2006;8(1):37-47.
63. Wilens TE, Biederman J, Spencer TJ, et al. A pilot controlled clinical trial of ABT-418, a cholinergic agonist, in the treatment of adults with attention deficit hyperactivity disorder. Am J Psychiatry. 1999;156(12):1931-1937.
64. Wilens TE, Verlinden MH, Adler LA, Wozniak PJ, West SA. ABT-089, a neuronal nicotinic receptor partial agonist, for the treatment of attention-deficit/hyperactivity disorder in adults: results of a pilot study. Biol Psychiatry. 2006;59(11):1065-1070.
65. Schultz R. Developmental deficits in social perception in autism: the role of the amygdala and fusiform face area. Int J Dev Neurosci. 2005;23(2-3):125-141.
66. Perry EK, Lee ML, Martin-Ruiz CM, et al. Cholinergic activity in autism: abnormalities in the cerebral cortex and basal forebrain. Am J Psychiatry. 2001;158(7):1058-1066.
67. Ray M, Graham AJ, Lee M, et al. Neuronal nicotinic acetylcholine receptor subunits in autism: an immunohistochemical investigation in the thalamus. Neurobiol Dis. 2005;19(3):366-377.
68. Lee M, Martin-Ruiz C, Graham A, et al. Nicotinic receptor abnormalities in the cerebellar cortex in autism. Brain. 2002;125(pt 7):1483-1495.
69. Lippiello PM. Nicotinic cholinergic antagonists: a novel approach for the treatment of autism. Med Hypotheses. 2006;66(5):985-990.
70. Hardan A, Handen BL. A retrospective open trial of adjunctive donepezil in children and adolescents with autistic disorder. J Child Adolesc Psychopharmacol. 2002;12(3):237-241.
71. Hertzman M. Galantamine in the treatment of adult autism: a report of three clinical cases. Int J Psychiatry Med. 2003;33(4):395-398.
72. Nicolson R, Craven-Thuss B, Smith J. A prospective, open-label trial of galantamine in autistic disorder. J Child Adolesc Psychopharmacol. 2006;16(5):621-629.
73. Chez M, Aimonovitch M, Buchanan T, Mrazek S, Tremb RJ. Treating autistic spectrum disorders in children: utility of the cholinesterase inhibitor rivastigmine tartrate. J Child Neurol. 2004;19(3):165-169.
74. Barker W, Luis CA, Kashuba A, et al. Relative frequencies of Alzheimer disease, Lewy body, vascular and frontotemporal dementia, and hippocampal sclerosis in the State of Florida Brain Bank. Alzheimer Dis Assoc Disord. 2002;16(4):203-212.
75. Delacourte A, Defossez A. Alzheimer’s disease: tau proteins, the promoting factors of microtubule assembly, are major components of paired helical filaments. J Neurol Sci. 1986;76(2-3):173-186.
76. Coyle J, Price DL, DeLong MR. Alzheimer’s disease: a disorder of cortical cholinergic innervation. Science. 1983;219(4589):1184-1190.
77. Warpman U, Nordberg A. Epibatidine and ABT 418 reveal selective losses of alpha 4 beta 2 nicotinic receptors in Alzheimer brains. Neuroreport. 1995;6(17):2419-2423.
78. Hogg RC, Bertrand D. Partial agonists as therapeutic agents at neuronal nicotinic acetylcholine receptors. Biochem Pharmacol. 2007;73(4):459-468.
79. Newhouse PA, Sunderland T, Tariot PN, et al. Intravenous nicotine in Alzheimer’s disease: a pilot study. Psychopharmacology (Berl). 1988;95(2):171-175.
80. Wilson AL, Langley LK, Monley J, et al. Nicotine patches in Alzheimer’s Disease: pilot study on learning memory and safety. Pharmacol Biochem Behav. 1995;51(2-3):509-514.
81. White HK, Levin ED. Four-week nicotine skin patch treatment effects on cognitive performance in Alzheimer’s disease. Psychopharmacology (Berl). 1999;143(2):158-165.
82. Potter A, Corwin J, Lang J, Piasecki M, Lenox R, Newhouse PA. Acute effects of the selective cholinergic channel activator (nicotinic agonist) ABT-418 in Alzheimer’s disease. Psychopharmacology (Berl). 1999;142(4):334-342.
83. Sahakian B, Jones G, Levy R, Gray J, Warburton D. The effects of nicotine on attention, information processing, and short-term memory in patients with dementia of the Alzheimer type. Br J Psychiatry. 1989;154:797-800.
84. Jones GM, Sahakian BJ, Levy R, Warburton DM, Gray JA. Effects of acute subcutaneous nicotine on attention, information processing and short-term memory in Alzheimer’s disease. Psychopharmacology (Berl). 1992;108(4):485-494.
85. van Duijn CM, Hofman A. Relation between nicotine intake and Alzheimer’s disease. BMJ. 1991;302(6791):1491-1494.
86. Brenner DE, Kukull WA, van Belle G, et al. Relationship between cigarette smoking and Alzheimer’s disease in a population-based case-control study. Neurology. 1993;43(2):293-300.
87. Graves AB, van Duijn CM, Chandra V, et al. Alcohol and tobacco consumption as risk factors for Alzheimer’s disease: a collaborative re-analysis of case-control studies. EURODEM Risk Factors Research Group. Int J Epidemiol. 1991;20 suppl 2:S48-S57.
88. Aggarwal NT, Bienias JL, Bennett DA, et al. The relation of cigarette smoking to incident Alzheimer’s disease in a biracial urban community population. Neuroepidemiology. 2006;26(3):140-146.
89. FDA Patient Safety News: Show #22, December 2003. New Class of Antibiotics for Skin Infections. Available at: www.accessdata.fda.gov/psn/printer-full.cfm?id=26. Accessed December 9, 2009.
90. Buisson B, Bertrand D. Open-channel blockers at the human alpha4beta2 neuronal nicotinic acetylcholine receptor. Mol Pharmacol. 1998;53(3):555-563.
91. Blanchard A, Guillemette G, Boulay G. Memantine potentiates agonist-induced Ca2+ responses in HEK 293 cells. Cell Physiol Biochem. 2008;22(1-4):205-214.
92. Kitagawa H, Takenouchi T, Azuma R, et al. Safety, pharmacokinetics, and effects on cognitive function of multiple doses of GTS-21 in healthy, male volunteers. Neuropsychopharmacology. 2003;28(3):542-551.
93. Timmermann D, Grønlien JH, Kohlhaas KL, et al. An allosteric modulator of the alpha7 nicotinic acetylcholine receptor possessing cognition-enhancing properties in vivo. J Pharmacol Exp Ther. 2007;323(1):294-307.
94. Glosser G. Neurobehavioral aspects of movement disorders. Neurol Clin. 2001;19(3):535-551.
95. Quik M, Bordia T, O’Leary K. Nicotinic receptors as CNS targets for Parkinson’s disease. Biochem Pharmacol. 2007;74(8):1224-1234.
96. Olanow C, Tatton WG. Etiology and pathogenesis of Parkinson’s disease. Annu Rev Neurosci. 1999;22:123-144.
97. Bohr I, Ray MA, McIntosh JM, et al. Cholinergic nicotinic receptor involvement in movement disorders associated with Lewy body diseases. An autoradiography study using [125I]aconotoxinMII in the striatum and thalamus. Exp Neurol. 2005;191(2):292-300.
98. Quik M, Bordia T, Forno L, McIntosh JM. Loss of a-conotoxinMII- and A85380-sensitive nicotinic receptors in Parkinson’s disease striatum. J Neurochem. 2004;88(3):668-679.
99. Gotti C, Moretti M, Bohr I, et al. Selective nicotinic acetylcholine receptor subunit deficits identified in Alzheimer’s disease. Parkinson’s disease and dementia with Lewy bodies by immunoprecipitation. Neurobiol Dis. 2006;23(2):481-489.
100. Forno LS. Neuropathology of Parkinson’s disease. J Neuropathol Exp Neurol. 1996;55(3):259-272.
101. Hong DP, Fink AL, Uversky VN. Smoking and Parkinson’s disease: does nicotine affect [alpha]-synuclein fibrillation? Biochim Biophys Acta. 2009;1794(2):282-290.
102. Quik M. Smoking, nicotine and Parkinson’s disease. Trends Neurosci. 2004;27(9):561-568.
103. Gentry C, Lukas RJ. Regulation of nicotinic acetylcholine receptor numbers and function by chronic nicotine exposure. Curr Drug Targets CNS Neurol Disord. 2002;1(4):359-385.
104. Fagerström K, Pomerleau O, Giordani B, Stelson F. Nicotine may relieve symptoms of Parkinson’s disease. Psychopharmacology (Berl). 1994;116(1):117-119.
105. Kelton MC, Kahn HJ, Conrath CL, Newhouse PA. The effects of nicotine on Parkinson’s disease. Brain Cogn. 2000; 43(1-3):274-282.
106. Ishikawa A, Miyatake T. Effects of smoking in patients with early-onset Parkinson’s disease. J Neurol Sci. 1993;117(1-2):28-32.
107. Clemens P, Baron JA, Coffey D, Reeves A. The short-term effect of nicotine chewing gum in patients with Parkinson’s disease. Psychopharmacology (Berl). 1995;117(2):253-256.
108. Vieregge A, Sieberer M, Jacobs H, Hagenah JM, Vieregge P. Transdermal nicotine in PD:a randomized, double-blind, placebo-controlled study. Neurology. 2001;57(6):1032-1035.
109. Ebersbach G, Stöck M, Müller J, Wenning G, Wissel J, Poewe W. Worsening of motor performance in patients with Parkinson’s disease following transdermal nicotine administration. Mov Disord. 1999;14(6):1011-1013.
110. Dickerson T, Janda KD. Glycation of the amyloid beta-protein by a nicotine metabolite: a fortuitous chemical dynamic between smoking and Alzheimer’s disease. Proc Natl Acad Sci U S A. 2003;100(14):8182-8187.
111. Buccafusco J, Terry AV Jr. The potential role of cotinine in the cognitive and neuroprotective actions of nicotine. Life Sci. 2003;72(26):2931-2942.
112. Quik M, Cox H, Parameswaran N, O’Leary K, Langston JW, Di Monte D. Nicotine reduces levodopa-induced dyskinesias in lesioned monkeys. Ann Neurol. 2007;62(6):588-596.
113. Parkinson Study Group. Randomized placebo-controlled study of the nicotinic agonist SIB-1508Y in Parkinson disease. Neurology. 2006;66(3):408-410.
114. Yanagida T, Takeuchi H, Kitamura Y, et al. Synergistic effect of galantamine on nicotine-induced neuroprotection in hemiparkinsonian rat model. Neurosci Res. 2008;62(4):254-261.
115. Sanberg P, Silver AA, Shytle RD, et al. Nicotine for the treatment of Tourette’s syndrome. Pharmacol Ther. 1997;74(1):21-25.
116. McEvoy J, Allen TB. The importance of nicotinic acetylcholine receptors in schizophrenia, bipolar disorder and Tourette’s syndrome. Curr Drug Targets CNS Neurol Disord. 2002;1(4):433-442.
117. Sanberg P, McConville BH, Fogelson HM, et al. Nicotine potentiates the effects of haloperidol in animals and patients with Tourette syndrome. Biomed Pharmacother. 1989;43(1):19-23.
118. McConville BJ, Sanberg PR, Fogelson MH, et al. The effects of nicotine plus haloperidol compared to nicotine only and placebo nicotine only in reducing tic severity and frequency in Tourette’s disorder. Biol Psychiatry. 1992;31(8):832-840.
119. Silver A, Shytle RD, Philipp MK, Wilkinson BJ, McConville B, Sanberg PR. Transdermal nicotine and haloperidol in Tourette’s disorder: a double-blind placebo-controlled study. J Clin Psychiatry. 2001;62(9):707-714.
120. Arevalo E, Licamele WL, Bronheim S, Sonnenschein K. Nicotine gum in Tourette’s disorder. Am J Psychiatry. 1992;149(3):417-418.
121. Shytle R, Silver AA, Philipp MK, McConville BJ, Sanberg PR. Transdermal nicotine for Tourette’s Syndrome. Drug Dev Res. 1996;38(3-4):290-298.
122. Silver A, Shytle RD, Sheehan KH, Sheehan DV, Ramos A, Sanberg PR. Multicenter, double-blind, placebo-controlled study of mecamylamine monotherapy for Tourette’s disorder. J Am Acad Child Adolesc Psychiatry. 2001;40(9):1103-1110.
123. Shytle RD, Silver AA, Lukas RJ, Newman MB, Sheehan DV, Sanberg PR. Nicotinic acetylcholine receptors as targets for antidepressants. Mol Psychiatry. 2002;7(6):525-535.
124. Woznica AA, George TP. Exploiting nicotinic receptor mechanisms for the treatment of schizophrenia and depression. J Dual Diagnosis. In press.